Guidelines to patient preparation for sample collection for three-glass (two-glass) urine test

  1. On the day before urine sample collection, stop physical exercises and loads. It is not recommended to eat vegetables and fruits that may cause urine too change color (beats, carrots etc.), do not take diuretics.
  2. It is recommended to refrain from collecting urine five to seven days after performing a cystoscopy, and to collect urine from menstruating women.
  3. To collect urine, use special containers available in pharmacies. Using such containers will prevent the ingress of foreign substances and increase the useful life of the sample.
  4. The container holding the biomaterial shall be labeled (special barcode of the patient shall be applied) for inpatients, or shall bear a label stating full name of the patient, their birth date, the name of the department and full name of the physician -- for outpatients. The container must bear the sample number -- No. 1 and No. 2 for the two-glass test or No. 1, No. 2 and No. 3 for the three-glass urine test.
  5. For a three-glass sampling, morning urine is collected as follows: in the morning, on an empty stomach after waking up and washing external genital organs thoroughly, the patient starts urinating into the first glass, continues urinating into the second glass and completes urination into the third glass. The second portion should be the largest in volume. When a third-glass sample is collected from a man, the last portion of urine is collected after a massage of the prostate gland. All the containers should be prepared beforehand, all must be labeled with the portion number.
  6. In urinology studies of women’s urine, the urine sample is most often taken in two portions. It is important that the first portion has small volume.
  7. Samples shall be delivered to the laboratory no later than 2 hours after taking.
  8. To prevent inaccurate analysis results, it is recommended not to perform different types of urine tests on the same day.