Guidelines to patient preparation for sample collection for 24-hour urine analyte test

  1. Urine is collected during a 24-hour period.
  2. Urine sample collection during menstruation or 5 to 7 days following cystoscopy is not allowed.
  3. After getting up in the morning, the patient shall void the bladder in the toilet and mark date and time (e.g., 5.10.19., 7:00).
  4. All the following portions of urine during the day and night, and the morning portion of urine of the following day are collected into the same container with a tight cap. It is recommended to make 100 ml marks on the container.
  5. Following the completion of urine collection, the daily volume of urine shall be determined. After that, urine shall be mixed in the closed container and no less than 50 ml into a prepared container of a smaller volume shall be poured immediately after that. This smaller container shall be delivered to the laboratory. Remaining urine shall be discarded.
  6. The container holding the biomaterial shall be labeled (special barcode of the patient shall be applied) for inpatients, or shall bear a label stating full name of the patient, their birth date, the name of the department and full name of the physician -- for outpatients.
  7. The measured daily urine volume (output) must be stated (in milliliters), e.g.: “Urine output: 1,200 ml”.
  8. When collecting a urine sample for microalbuminuria testing, try to prevent any factors that may distort study results. Urine albumin level is increased in cases of an infection, poor compensation of diabetes mellitus, noncompensated arterial hypertension, and is decreased by the administration of non-steroidal anti-inflammatory pharmaceuticals, angiotensin-converting enzyme inhibitors or angiotensin II receptor blockers.
  9. To prevent inaccurate results, it is not recommended to collect urine samples for a common analysis, 24-hour analysis and for the Nechiporenko urine test on the same day!