Guidelines of patient preparation for blood collection for hormonal analysis, cancer-specific markers and infection markers
Guidelines of patient preparation for blood collection for hormonal analysis, cancer-specific markers and infection markers

  1. On the day before taking a blood sample for hormonal analysis, cancer-specific markers and infection markers, physical exercises, stress, physiotherapy procedures, administration of pharmaceuticals (with the exclusion of cases of pharmacological therapy prescribed by a physician; in this case the decision to suspend medication shall be made by the consulting physician), administration of oral contraceptives, drinking alcoholic beverages and eating fatty food shall be excluded. Immediately before the test, smoking is forbidden.
  2. Standard blood sampling is performed in the morning, on an empty stomach (no less than 8 to 12 hours after the previous intake of food), in a procedure room, in a sitting or lying position, in a physiologically calm state, from an ulnar vein, following all the aseptic and antiseptic regulations. Prior to blood sampling, the patient shall rest for 15 minutes.
  3. If thyroid gland function studies are performed during a period when thyroid gland hormone drugs are administered, they shall commence 24 hours after the last intake of the drug. Two to three days before blood sampling, administration of pharmaceuticals containing iodine shall be suspended.
  4. When testing for ACTH, it is recommended to perform blood sampling in the morning (7:00 to 9:00). This requirement is due to the fact that reference values for the hormone were estimated for this period.
  5. When testing for cortisol, blood shall be sampled before 10:00.
  6. When studying sex hormones in women during the reproductive period: on day 3 to 5 of the menstrual cycle (FSH, LH, estradiol, prolactin), on day 21 of the menstrual cycle (it is advisable to test for progesteron as late as possible in the second half of the menstrual cycle, that is, on day 20 to 23 of the regular 28-30-day cycle).
  7. When studying sex hormones in women during the reproductive period, the referral shall state the date and time of blood sampling, the day and phase of the menstrual cycle, or -- in case of pregnancy -- the gestational age.
  8. Tests for testotsteron and dehydroepiandrosterone (DHEA-S) shall be performed only subject to relevant clinical indications (signs of hirsutism, androgenital syndrome, etc.). It is recommended to perform these studies during the first patient’s visit (e.g., on day 3 to 5 of the cycle after it was noticed).
  9. In cases of infertility, the above tests are mandatory. This also includes a mandatory assessment of thyroid gland function.
  10. During the menopause, it is not effective to perform progesteron, cortisol, testosteron and dehydroepiandrosterone (DHEA-S) level tests (unless there are clinical indications).
  11. To ensure higher accuracy and better interpretation of results, the tests are recommended to be performed not once, but repeatedly after some time. Repeated tests shall be performed at the same time of day, two to three weeks later. Analysis of results of studies performed over time is more informative, from both diagnostic and prognostic point of view.
  12. If it is impossible to collect samples for hormone testing at specific days of the cycle, it is best not to perform sampling since the analysis would be absolutely non-diagnostic.
  13. PSA (total and free): blood sampling shall be performed no sooner than two weeks following prostate gland biopsy and prostate massage; the postoperative level shall be determined no sooner than 6 weeks after intervention.
  14. It is more diagnostically accurate to perform СА-125 sampling two to three days after the termination of menstruation.
  15. Comprehensive studies (multiple tests performed over a single sample) are more informative.
  16. If the study musts be performed during the course of a pharmaceutical therapy, the referral shall state the name of the pharmaceutical and the time of its latest administration.
  17. Blood studies to reveal infections: two days before sampling for virus hepatitis, refrain from eating citrus fruits, orange-colored fruits and vegetables; IgM type antibodies blood testing shall be performed no sooner than 5 to 7 days after the contraction of the disease; IgG, IgA type antibodies blood testing shall be performed no sooner than 10 to 14 days after the contraction of the disease -- please contact your physician! In case of doubt, a repeated study shall be performed in two or three weeks.
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