INDICATIONS FOR PCR DIAGNOSTICS

The PCR technique has become a “golden standard” in the diagnosis of many disorders. PCR diagnostics means:

• direct detection of the disease agent in the studied sample;
• high specificity enabling the location of the unique DNA or RNA region;
• high sensitivity of the technique enabling the detection of even singular originator cells (viruses and bacteria);
• the procedure makes it possible to detect multiple disease agents using the same sample;
• it is possible to detect pathogens even before any symptoms of the disease develop (so-called symptomless diagnostics) and after the disease has been cured (retrospective diagnostics).

OUR LABORATORY PERFORMS:

• PCR-based real-time detection of DNA of Chlamydia trachomatis microbes.
• PCR-based real-time detection of DNA of Chlamydia trachomatis microbes in prostate gland fluid.
• PCR-based real-time detection of DNA of Mycoplasma genitalium microbes.
• PCR-based real-time detection of DNA of Ureaplasma urealyticum microbes.
• PCR-based real-time detection of DNA of Trichomonas vaginalis microbes.
• PCR-based real-time detection of DNA of Trichomonas vaginalis microbes in prostate gland fluid.
• PCR-based real-time detection of DNA of Gardnerella vaginalis microbes.
• PCR-based real-time quantitative estimation of Ureaplasma parvum, Ureaplasma urealyticum and Mycoplasma hominis.
• PCR-based real-time quantitative estimation of human papilloma virus DNA (HPV VCR 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68) (screening tests for women and men). 
• PCR-based real-time detection of DNA of type 1 and 2 Herpes simplex microbes.
• PCR-based real-time detection of DNA of Cytomegalovirus microbes.
• PCR-based real-time quantitative estimation of Candida family fungi (Candida albicans, Candida glabrata, Candida krusei, Candida parapsilosis and Candida tropicalis).
• Aerobic bacteria Detection of enterobacteria DNA, staphylococcus DNA, streptococcus DNA.
• Florocenosis Bacterial vaginosis Estimation of the number of bacteria, DNA of lactobacilli, DNA of gardnerelli, DNA of atopobiums.
• Florocenosis Mycoplasma. PCR-based real-time quantitative estimation of Ureaplasma parvum, Ureaplasma urealyticum and Mycoplasma hominis (in women).
• PCR-based real-time detection of DNA of Mycoplasmapneumoniae and Chlamydophila pneumoniae.
• PCR-based real-time detection of disease agents of acute respiratory infections (hRSv, hMpv, hPiv, hCov, hRv, etc.).
• PCR-based real-time detection of Influenza virus A and Influenza virus В.
• PCR-based real-time HLA matching (H1N1 and H3N2 sub-type identification) of Influenza virus A in clinical material.
• PCR-based real-time detection and quantitative estimation of DNA of Epstein-Barr virus (EFV), Cytomegalovirus and type 6 herpes viruses.
• PCR-based real-time detection of DNA of N.gonorrhoeae, C.trachomatis, M.genitalium and T. Vaginalis (using the NCMT test kits).
• PCR-based real-time detection of DNA of Chlamydia trachomatis U.parvum, U.urealyticum, M.genitalium and M.hominis.
• PCR-based real-time detection of DNA of type B hepatitis (HBV) microbes.
• PCR-based real-time quantitative estimation of DNA of type B hepatitis (HBV) microbes.
• PCR-based real-time detection of DNA of type B hepatitis (HCV) microbes.
• PCR-based real-time quantitative estimation of DNA of type C hepatitis (HCV) microbes.
• PCR-based real-time detection of type C hepatitis (HCV) and identification of its genotype.

Microbiota research

• Femoflor® Screen - screening study of the microflora of the urogenital tract in women (13 indicators)
• Femoflor® 16 - study of the biocenosis of the urogenital tract in women (17 indicators)
• Androflor® Screen - study of the microflora of the urogenital tract of men (15 indicators)
• Androflor® - study of the microflora of the urogenital tract of men (24 indicators)
• HPV quantum-21 - identification, typing and quantitative determination of human papillomavirus (21 indicators)

Genetics

• Genetics of folate metabolism (determination of polymorphisms in the MTHFR, MTR, MTRR genes associated with folate cycle disorders)
• Genetics of hemostasis (determination of gene polymorphisms associated with disorders of the blood coagulation system F2, F5, F7, F13, FGB, ITGA2, ITGB3, SERPINE1)

OncoGenetics BRCA

Fungal infections

Nosocomial and community-acquired infections

• Detection of DNA of opportunistic bacteria of the classes Bacilli, Betaproteobacteria and Gammaproteobacteria, causing nosocomial and community-acquired infections.
• Identification of resistance genes to glycopeptide and beta-lactam antibiotics: van A\B (vancomycin, teicoplanin); mec A (methicillin, oxacillin); tem, ctx-M-1, shv (penicillins and cephalosporins); oxa-40-like, oxa-48-like, oxa-23-like, oxa-51-like, imp, kpc, ges, ndm, vim (carbapenems).

PREPARATION FOR PCR DIAGNOSTICS

Guidelines of patient preparation for sample collection for real-time PCR test

HOW DO I GET MY TEST TAKEN?

1. You need to complete and sign a paid service contract at the Reception or, if you have a physician's referral, present it to the receptionist.
2. Pay the bill at the RCMC pay office, or through the ERIP electronic payment system.
3. Have your sample taken.

Test material is accepted according to schedule (Friday through Monday), from 8:00 till 10:00. Test results are made available to the physician and the patient at 15:00 on the same day.